RAPGEF2 Knockout cell line (A549)

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location： Home > Products > KO Cell Lines > RAPGEF2 Knockout cell line (A549)

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RAPGEF2 Knockout cell line (A549) Order

RAPGEF2 Knockout cell line (A549)
Catalog#
YKO-HS557
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
RAPGEF2 Knockout cell line (A549)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
RAPGEF2 Knockout cell line (A549)
Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:4
Species
Human
Gene Name
RAPGEF2
Gene ID
9693
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90% F12K+10% FBS
Target region
Knockout region: Exon 9 (based on transcript RAPGEF2-216)
Validation: PCR+Sanger Sequencing
RAPGEF2 Knockout cell line (A549) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:A549		 STR Info (Cell bank)
Cell Line:A-549
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11111111
D13S317 11111111
D7S820 811811
D16S539 11121112
VWA 14141414
TH01 89.389.3
AMEL XYXY
TPOX 811811
CSF1PO 10121012
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the RAPGEF2 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the A549 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, RAPGEF2 gene knockout RAPGEF2 Knockout cell line (A549) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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